Water permeability differs between growing and non-growing barley leaf tissues
Identifieur interne : 001441 ( Main/Exploration ); précédent : 001440; suivant : 001442Water permeability differs between growing and non-growing barley leaf tissues
Auteurs : Vadim Volkov [Royaume-Uni] ; Charles Hachez [Belgique] ; Menachem Moshelion [Israël] ; Xavier Draye [Belgique] ; Franois Chaumont [Belgique] ; Wieland Fricke [Royaume-Uni, Irlande (pays)]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2006-11-22.
Abstract
A pressure probe technique and an osmotic swelling assay were used to compare water transport properties between growing and non-growing tissues of leaf three of barley. The epidermis was analysed in planta by pressure probe, whereas (predominantly) mesophyll protoplasts were analysed by osmotic swelling. Hydraulic conductivity (Lp) and, by implication, water permeability (Pf) of epidermal cells was 31% higher in the leaf elongation zone (Lp0.50.2 m s1 MPa1; Pf6525 m s1; means SD of n17 cells) than in the, non-growing, emerged leaf zone (Lp0.40.1 m s1 MPa1; Pf5015 m s1; n24; P <0.05). Similarly, water permeability of mesophyll protoplasts was by 55% higher in the elongation compared with emerged leaf zone (Pf131 m s1 compared with 81 m s1; n57 and 36 protoplasts, respectively; P <0.01). Within the leaf elongation zone, a small population of larger-sized protoplasts could be distinguished. These protoplasts, which originated most likely from parenchymateous bundle sheath or midrib parenchyma cells, had a three-fold higher water permeability (P <0.001) as mesophyll protoplasts. The effect on Lp and Pf of known aquaporin inhibitors was tested with the pressure probe (Au, Ag, Hg2, phloretin) and the osmotic swelling assay (phloretin). Only phloretin, when applied to protoplasts in the swelling assay caused an average decrease in Pf, but the effect varied between isolations. Technical approaches and cell-type and growth-specific differences in water transport properties are discussed.
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DOI: 10.1093/jxb/erl203
Affiliations:
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The epidermis was analysed in planta by pressure probe, whereas (predominantly) mesophyll protoplasts were analysed by osmotic swelling. Hydraulic conductivity (Lp) and, by implication, water permeability (Pf) of epidermal cells was 31% higher in the leaf elongation zone (Lp0.50.2 m s1 MPa1; Pf6525 m s1; means SD of n17 cells) than in the, non-growing, emerged leaf zone (Lp0.40.1 m s1 MPa1; Pf5015 m s1; n24; P <0.05). Similarly, water permeability of mesophyll protoplasts was by 55% higher in the elongation compared with emerged leaf zone (Pf131 m s1 compared with 81 m s1; n57 and 36 protoplasts, respectively; P <0.01). Within the leaf elongation zone, a small population of larger-sized protoplasts could be distinguished. These protoplasts, which originated most likely from parenchymateous bundle sheath or midrib parenchyma cells, had a three-fold higher water permeability (P <0.001) as mesophyll protoplasts. The effect on Lp and Pf of known aquaporin inhibitors was tested with the pressure probe (Au, Ag, Hg2, phloretin) and the osmotic swelling assay (phloretin). Only phloretin, when applied to protoplasts in the swelling assay caused an average decrease in Pf, but the effect varied between isolations. Technical approaches and cell-type and growth-specific differences in water transport properties are discussed.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>Irlande (pays)</li><li>Israël</li><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Volkov, Vadim" sort="Volkov, Vadim" uniqKey="Volkov V" first="Vadim" last="Volkov">Vadim Volkov</name></noRegion><name sortKey="Fricke, Wieland" sort="Fricke, Wieland" uniqKey="Fricke W" first="Wieland" last="Fricke">Wieland Fricke</name><name sortKey="Fricke, Wieland" sort="Fricke, Wieland" uniqKey="Fricke W" first="Wieland" last="Fricke">Wieland Fricke</name></country><country name="Belgique"><noRegion><name sortKey="Hachez, Charles" sort="Hachez, Charles" uniqKey="Hachez C" first="Charles" last="Hachez">Charles Hachez</name></noRegion><name sortKey="Chaumont, Franois" sort="Chaumont, Franois" uniqKey="Chaumont F" first="Franois" last="Chaumont">Franois Chaumont</name><name sortKey="Draye, Xavier" sort="Draye, Xavier" uniqKey="Draye X" first="Xavier" last="Draye">Xavier Draye</name></country><country name="Israël"><noRegion><name sortKey="Moshelion, Menachem" sort="Moshelion, Menachem" uniqKey="Moshelion M" first="Menachem" last="Moshelion">Menachem Moshelion</name></noRegion></country><country name="Irlande (pays)"><noRegion><name sortKey="Fricke, Wieland" sort="Fricke, Wieland" uniqKey="Fricke W" first="Wieland" last="Fricke">Wieland Fricke</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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